Detailed view for Tb927.1.1560

Basic information

TDR Targets ID: 20350
Trypanosoma brucei, vesicular-fusion protein nsf, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.3696 | Length (AA): 725 | MW (Da): 79954 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005488   binding  
GO:0000166   nucleotide binding  
GO:0017111   nucleoside-triphosphatase activity  
GO:0016887   ATPase activity  
GO:0005524   ATP binding  
GO:0006886   intracellular protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 180 1qcs (A) 2 197 23.00 0 1 0.549776 -0.48
53 689 3cf2 (A) 77 730 25.00 0 1 0.989121 1.13
204 469 3h4m (A) 172 411 42.00 0 1 0.769297 -0.18
387 467 2dzn (D) 349 412 22.00 0 0.88 0.170224 -0.47
487 699 1d2n (A) 514 727 38.00 0 1 0.875193 -1.17
532 722 4ww0 (A) 202 405 17.00 0.51 1 0.445848 0.13
641 722 3pfi (A) 170 253 16.00 0.2 0.16 0.221503 -0.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127665)

Species Accession Gene Product
Arabidopsis thaliana AT4G04910   N-ethylmaleimide sensitive factor
Babesia bovis BBOV_II007200   N-ethylmaleimide-sensitive factor, putative
Brugia malayi Bm1_27160   vesicle-fusing ATPase
Candida albicans CaO19.12461   similar to C terminus of S. cerevisiae ATPase SEC18 (YBR080C) involved in protein transport between ER and Golgi
Candida albicans CaO19.4993   similar to N terminus of S. cerevisiae ATPase SEC18 (YBR080C) involved in protein transport between ER and Golgi
Candida albicans CaO19.12460   similar to N terminus of S. cerevisiae ATPase SEC18 (YBR080C) involved in protein transport between ER and Golgi
Candida albicans CaO19.4994   similar to C terminus of S. cerevisiae ATPase SEC18 (YBR080C) involved in protein transport between ER and Golgi
Caenorhabditis elegans CELE_H15N14.2a   Protein NSF-1, isoform B
Caenorhabditis elegans CELE_H15N14.2   Protein NSF-1, isoform A
Cryptosporidium hominis Chro.20314   N-ethylmaleimide-sensitive factor; NSF; Sec18p
Cryptosporidium parvum cgd2_3010   N-ethylmaleimide-sensitive factor (NSF1)-like AAA ATpase involved in vesicular transport
Dictyostelium discoideum DDB_G0276153   N-ethylmaleimide-sensitive fusion protein
Drosophila melanogaster Dmel_CG1618   comatose
Drosophila melanogaster Dmel_CG33101   N-ethylmaleimide-sensitive factor 2
Echinococcus granulosus EgrG_000338600   vesicular fusion protein nsf
Entamoeba histolytica EHI_004640   vesicle-fusing ATPase, putative
Echinococcus multilocularis EmuJ_000338600   vesicular fusion protein nsf
Giardia lamblia GL50803_112681   NSF
Giardia lamblia GL50803_114776   NSF
Homo sapiens ENSG00000073969   N-ethylmaleimide-sensitive factor
Leishmania braziliensis LbrM.20.4990   vesicle-fusing ATPase, putative,N-ethylmaleimide-sensitive factor, putative
Leishmania donovani LdBPK_200820.1   vesicular-fusion protein nsf, putative
Leishmania infantum LinJ.20.0820   vesicle-fusing ATPase, putative,N-ethylmaleimide-sensitive factor, putative
Leishmania major LmjF.20.0810   N-ethylmaleimide-sensitive factor, putative
Leishmania mexicana LmxM.20.0810   vesicle-fusing ATPase, putative,N-ethylmaleimide-sensitive factor, putative
Loa Loa (eye worm) LOAG_10143   vesicle-fusing ATPase
Loa Loa (eye worm) LOAG_11814   vesicle-fusing ATPase
Mus musculus ENSMUSG00000034187   N-ethylmaleimide sensitive fusion protein
Neospora caninum NCLIV_011040   N-ethylmaleimide-sensitive factor, putative
Oryza sativa 4339340   Os05g0519400
Plasmodium berghei PBANKA_0401600   N-ethylmaleimide-sensitive fusion protein, putative
Plasmodium falciparum PF3D7_0303000   N-ethylmaleimide-sensitive fusion protein
Plasmodium knowlesi PKNH_0840100   N-ethylmaleimide-sensitive fusion protein, putative
Plasmodium vivax PVX_119275   N-ethylmaleimide-sensitive fusion protein, putative
Plasmodium yoelii PY05628   ATPase, AAA family, putative
Saccharomyces cerevisiae YBR080C   AAA family ATPase SEC18
Schistosoma japonicum Sjp_0210580   ko:K01509 adenosinetriphosphatase [EC3.6.1.3], putative
Schistosoma mansoni Smp_057320   vesicular-fusion protein nsf
Schmidtea mediterranea mk4.000372.04   Vesicle-fusing ATPase
Schmidtea mediterranea mk4.000372.03  
Trypanosoma brucei gambiense Tbg972.1.790   N-ethylmaleimide sensitive factor (NsF),vesicular-fusion protein nsf, putative
Trypanosoma brucei Tb927.1.1560   vesicular-fusion protein nsf, putative
Trypanosoma congolense TcIL3000_1_910   vesicular-fusion protein nsf, putative
Trypanosoma cruzi TcCLB.508995.40   vesicular-fusion protein nsf, putative
Trypanosoma cruzi TcCLB.506477.20   vesicular-fusion protein nsf, putative
Toxoplasma gondii TGME49_318510   N-ethylmaleimide-sensitive fusion protein, putative
Theileria parva TP02_0172   N-ethylmaleimide sensitive protein, putative
Trichomonas vaginalis TVAG_476020   vesicular-fusion protein nsf, putative

Essentiality

Tb927.1.1560 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.1560 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.1.1560 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.1560 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.1560 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_H15N14.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_H15N14.2 Caenorhabditis elegans larval arrest wormbase
CELE_H15N14.2 Caenorhabditis elegans larval lethal wormbase
CELE_H15N14.2 Caenorhabditis elegans slow growth wormbase
CELE_H15N14.2 Caenorhabditis elegans sterile wormbase
YBR080C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0401600 Plasmodium berghei Essential plasmo
TGME49_318510 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell morphogenesis (GO:0000902) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell cycle (GO:0007049) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
mitochondrion organization and biogenesis (GO:0007005) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
endocytosis (GO:0006897) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.1.1560 (Trypanosoma brucei), vesicular-fusion protein nsf, putative
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